Clinical Chemistry Study Guide
Direct antigen detection of viruses by immunoassays are increasingly common. The Directigen Flu A test is a 15min test for influenza virus. What type of monoclonal antibodies are used in this test? Two monoclonal antibody conjugates specific for influenza A nucleoproteins b. Two monoclonal antibody conjugates specific for influenza A surface HA proteins c. Polyclonal antibody conjugates specific for influenza A nucleoproteins d. Polyclonal antibody conjugates specific for influenza A surface HA protein.
RF testing is typically performed to assist in the clinical diagnosis of RA. Which is not an assay used clinically to detect RF in serum?
ELISA test with serum sample being added to purified RF antigen which is bound to the microtiter plate b. Nephelometry test with serum sample being mixed with test reagent (antibodies) that cause the blood to clump if RF is present c. Agglutination test with serum sample being mixed with IgG-coated latex beads that cause the blood to clump if RF is present d. Microscopic analysis of Hep-2 cells (fixed to microscope plate) after addition of serum sample and fluorescence-labeled antibodies against human IgG. Prostate Specific Antigen (PSA) can bind two different proteins in the blood (antichymotrypsin and alpha macroglobulin) in the following ratios: a. Benign prostate conditions result in more unbound PSA, and prostate cancer conditions result in more bound PSA.
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- Principles of medical decision making, (patho)physiology, organ function, analytical techniques, clinical cases that illustrates how a laboratory contributes to diagnosis, prognosis and monitoring of disease are adressed.
Dec 3, 2013 - This is a fairly thorough without being bogged down with unnecessary detail study guide for Medical Laboratory Technician studying for the.
Benign prostate conditions result in more bound PSA, and prostate cancer conditions result in more unbound PSA. Benign and cancer prostate conditions both result in bound PSA.
Clinical Chemistry Study Guide
Benign and cancer prostate conditions both result in unbound PSA. Agglutination test -mix blood with human IgG bound to latex beads -beads clump (agglutinate) if RF postive -Screening test for RA OR -mix blood with a sheep's RBC covered with rabbit antibodies -if RF is present the RBC clump together -confirm presence of RF 2. Nephelometry test -mix blood with antibodies that cause the blood to clump if RF present -as levels of RF increase, more clumping occurs, causing a cloudier sample and less light to pass through the tube.measure turbidity. Qualitative Test: -add blood sample to HEP-2 cells (fixed to slide) which have 100 to 150 possible autoantigens -detect ANAs with fluorescence-labeled antibodies bound to cells in which both titer and pattern can be described using microscope Fluorescent ANA (FANA) test: -Serially dilute with saline to obtain titer (1/40, 1/80.1/640) -Evaluate under the microscope.Homogeneous-most frequent, but also drug induced lupus, RA.